University of Valencia logo Logo Central Service for Experimental Research (SCSIE) Logo del portal

Cell Culture laboratory

Cell Culture laboratory of Biological Containment Level 2 (BCL2). It has several rooms: Cell Lines Room, Primary Cultures Room, Hypoxia Room and Viral Vectors Room (BCL2+), with 10 biosafety cabinets, 6 incubators and all the necessary equipment to work with eukaryotic cell cultures.

Prepared and controlled by technicians so the user just focuses on his experiment.

Cell Separator Flow Cytometer ARIA FUSION

Cell Separator equipped with 6 lasers, biosafety cabinet and aerosol extraction. It allows, firstly, the rapid measurement of morphological and functional parameters of a large number of individual cells suspended in liquid medium. For this, cells are initially labelled with fluorochrome-conjugated antibodies, or fluorescent probes that bind to proteins located on the cell surface or inside the cell. The scattered light and fluorescence emitted by the markers as the cells are passed one by one through the different lasers allows simultaneous measurement of structural features and various parameters in each of these cells.

In addition, after analysis, it is possible to separate and collect subpopulations of interest from the sample for further studies.

Cryopreservation room

Cryopreservation room belonging to the Cell Culture laboratory for the storage and management of frozen samples from users and the section's cell bank.

Cytometry laboratory

Fully equipped laboratory for sample preparation, acquisition, separation and analysis by flow cytometry.

Flow Cytometer Analyser FORTESSA

A 5-laser analysing cytometer for rapid measurement of morphological and functional parameters of large numbers of single cells suspended in a liquid medium. For this purpose, cells are initially labelled with fluorochrome-conjugated antibodies or fluorescent probes that bind to proteins located on the cell surface or inside the cell. The scattered light and fluorescence emitted by the markers as the cells are passed one by one through the different lasers allows simultaneous measurement of structural features and various parameters in each of these cells.

Flow Cytometer Analyser VERSE

A three-laser analysing cytometer for rapid measurement of morphological and functional parameters of large numbers of single cells suspended in liquid medium. For this purpose, cells are initially labelled with fluorochrome-conjugated antibodies or fluorescent probes that bind to proteins located on the cell surface or inside the cell. The scattered light and fluorescence emitted by the markers as the cells are passed one by one through the different lasers allows simultaneous measurement of structural features and various parameters in each of these cells.

Hypoxia/hiperoxia room

Room located inside the Cell Culture laboratory (BCL2) composed of a closed Hypoxia Station and necessary equipment for the maintenance of the cultures. The closed station allows a precise control of the percentage of hypoxia and/or hyperoxia, even at extreme values, keeping the culture conditions constant at all times, both during its handling and within the incubators. All of this ensures reproducible data.

Microultracentrifuge

Ultracentrifuge for small volumes with 3 rotors: S55A2, S50A and swing-out rotor S52ST.

NCB2+ room for cell cultures

Room located inside the Cell Culture laboratory (NCB2), with its own equipment and higher level work protocols. Designed to work with viral vectors, GMOs or level 2 biological agents that require greater security.