GIUV2013-174

It is a research group dedicated to the study of tumours of the central nervous system, addressing clinical, neuropathological, genetic, molecular and metabolomic aspects. These studies are carried out in both "in vivo" tumours and "in vitro" experimental models, with a translational orientation with the aim of biopathological characterisation towards the identification of molecular targets with application to diagnosis, prognosis and therapeutics.

No Determinada

• The overall objective of this work is the clinical, histopathological, and genetic characterization of CNS tumors.
• Define the genetic and molecular profile that allows establishing diagnostic and prognostic criteria and proposing effective therapeutic actions.
• Analyze the epigenetic mechanisms linked to the initiation and progression processes through analysis of changes in gene methylation that have prognostic value.
• Analysis of microRNAs involved in the gene modulation of oncogenic cell functions (MET, cell migration, angiogenesis, apoptosis, cell cycle).
• Development and analysis of in vitro and in vivo experimental models (xenotransplants) with the aim of defining significant molecular targets in oncogenic processes that can be used as diagnostic, prognostic and therapeutic values.

• Primary GBM. EGFR amplification status and angiogenic / infiltrative phenotypes. Molecular networks..The aim of this work is to define the phenotypic and genetic characteristics of glioblastoma multiforme (GBM). To analyse the angiogenic and infiltrative characteristics of the nodular and diffuse pattern of GBM, the genetic profiles and the molecular networks responsible for these GBM patterns in relation to the EGFR receptor amplification profiles.
• Role of microRNA in regulating signaling pathways of EGFR..The aims of this work are to study the expression of miRNAs in high-grade gliomas, such as primary glioblastoma multiforme, secondary glioblastoma and high-grade astrocytoma.
• Development of a cell culture model of GBM, the modulation of gene activity by microRNAs..The aim of this work is to generate experimental models to study the modulation of gene activity by transfection methods (inhibition and overexpression) of miRNAs in primary cell cultures and established glioblastoma multiforme cell lines.
• Development of a model of population analysis and spatial distribution in these neoplasms.This line of work is aimed at establishing models of tumour growth in GBM through the analysis of cell populations in mitosis/cell cycle and their distribution in the tumour, establishing intercellular and intratumoural spatial patterns.
• Metabolomics and microvascular environment of aggressive human glioma characterization by DCE-MRI and genetic study of biopsies..The aims of this work are to characterise the microvascular environment of gliomas and to study the infiltration, migration and invasiveness of these neoplasms using DCE-MRI.
• Clinical, histopathological and genetic characterization in recurrent Meningioma.The aims of this work are to establish morphological and genetic profiles of recurrent meningioma with a diagnostic and prognostic objective.

• -

• Pathology

• EGFR
• MICROVASCULAR
• MENINGIOMA
• MLPA
• NODULAR AND DIFFUSE PATTERN
• GLIOMAS
• PRIMARY CULTURES OF GBM
• CELL LINES
• TRANSFECTION
• INHIBITORS
• NEOPLASMS
• INFILTRATION
• METABOLOMICS
• GBM
• EXPRESSION
• MIRNAS
• DISTRIBUTION
• POPULATION