Grups d'Investigació

GIUV2015-252

Self-assembly of amphipathic molecules with lipid and polypeptide characteristics, natural or synthetic. Characterisation of membrane structures of biological, biomedical and biotechnological interest, with special interest in pore, fusion and fission structures. Light-induced molecular changes in membranes, natural or synthetic. Design, implementation and applications of molecular switches in membranes.

https://www.uv.es/membiophys/

• Disseny i caracteritzacio de la interaccio amb membranes lipidiques de peptids i proteines amb activitat formadora de porus y remodelarora de membranas.
• Descripcio de mecanismes de formacio de porus, canvis de curvatura i processos de fusio i fissio de membranes.
• Determinants moleculars i propietats fisic-quimiques responsables a nivell de sequencia de de l'activitat en membranes de molecules polipeptdidicas.
• Redisseny de molecules polipeptidiques per al control de la seva activitat.
• Desenvolupament de nova metodologia, experimental i teorica, per a l'estudi estructural i funcional d'activitats de membrana.

• Design and synthesis of membrane-active polipeptides.Design of new polypeptide sequences with membrane activity from native proteins and peptides. Methodologies: Computational analysis of polypeptide structures at various levels. Chemical synthesis of peptides.
• Biophysical characterization of polypeptide-membrane complexes in model systems.Study of the kinetic and thermodynamic properties of membrane-active complexes. Description of the structure of active complexes and their formation mechanisms. Methodology: infrared, UV-Vis, DC and fluorescence spectroscopies. Fluorescence microscopy. AFM nanoscopy. Langmuir monocaps. Molecular simulations. Theoretical diffusion models. Statistical analysis.

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• Institute of Molecular Science (ICMOL)

• Pèptids membranoactius, dominios mínimos con actividad de membrana
• GUV, ATR-FTIR, Vesícula individual, Ley de Fick, Monocapas, Membranas soportadas