R&D+i of the Spanish Type Culture Collection (CECT): Microbiology at the service of Biotechnology - CECT I+D+i

Reference of the Group:

GIUV2023-555

 
Description of research activity:
The group's research activity aims to support the development of microbiological-based biotechnology by offering services to both the scientific community and the business sector. The group is linked to the Spanish Type Culture Collection (CECT), the only public Microbial Resource Centre (mBRC) in Spain that acts as a depository and supplier of bacteria, archaea, yeasts and filamentous fungi and offers microorganism identification and characterisation services, among others. The team of technicians that make up the CECT staff contributes their knowledge and experience in research related to the conservation of bacteria, archaea, yeasts and filamentous fungi; the identification and characterisation of microorganisms using classical microscopy and culture techniques and advanced molecular techniques (NGS, MALDI-TOF, FAME-MIDI); microbial taxonomy and the characterisation of strains to search for or improve biotechnological applications. In addition, it also provides support in legal aspects related to the use and management of strains, including compliance with the access and benefit-sharing regulations of the Nagoya Protocol, the deposit of microorganisms for patent purposes and...The group's research activity aims to support the development of microbiological-based biotechnology by offering services to both the scientific community and the business sector. The group is linked to the Spanish Type Culture Collection (CECT), the only public Microbial Resource Centre (mBRC) in Spain that acts as a depository and supplier of bacteria, archaea, yeasts and filamentous fungi and offers microorganism identification and characterisation services, among others. The team of technicians that make up the CECT staff contributes their knowledge and experience in research related to the conservation of bacteria, archaea, yeasts and filamentous fungi; the identification and characterisation of microorganisms using classical microscopy and culture techniques and advanced molecular techniques (NGS, MALDI-TOF, FAME-MIDI); microbial taxonomy and the characterisation of strains to search for or improve biotechnological applications. In addition, it also provides support in legal aspects related to the use and management of strains, including compliance with the access and benefit-sharing regulations of the Nagoya Protocol, the deposit of microorganisms for patent purposes and biosecurity issues.Our research covers, on the one hand, studies on the biodiversity of food lactic bacteria aimed at searching for strains with biotechnological application capabilities, including isolation, identification, genomic analysis and evaluation of food safety. On the other hand, it addresses the identification, detection and quantification using PCR and massive sequencing techniques of pathogens in food and water, including human enteric viruses and antibiotic-resistant bacteria, as well as their viable/infectious forms (vqPCR), and the validation of qPCR and vqPCR methods for the detection of pathogens in food and water matrices and their adaptation to routine analysis to evaluate the health risk and its involvement in food safety. In this regard, the research group has internationally recognised experts in microbial contaminants of emerging concern, including the SARS-CoV-2 pandemic, particularly focusing on the development of new omics and bioinformatics techniques for their identification and characterisation.
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Web:

https://www.uv.es/uvweb/coleccion-espanola-cultivos-tipo/es/coleccion-espanola-cultivos-tipo-1285872233521.html

 
Scientific-technical goals:
  • Identificacion, conservacion y valorizacion de recursos microbianos para aplicaciones biotecnologicas
  • Desarrollo de metodologias de deteccion, identificacion y cuantificacion de microorganismos de importancia en seguridad alimentaria
 
Research lines:
  • Characterization of lactic acid bacteria of agrofood interest.Characterisation of new lactic acid bacteria strains: identification and classification by molecular techniques and genomic sequencing. Study of their biotechnological potential and application in functional foods and agri-food.
  • Detection of microbial pathogens in food by PCR.Development of rapid real-time PCR-based methods for the quantitative detection of pathogens of interest in foods (enteric bacteria and viruses) as well as viable/infectious forms.
 
Group members:
Name Nature of participation Entity Description
ROSA AZNAR NOVELLADirectorUniversitat de València
Research team
GLORIA SANCHEZ MORAGASMemberUniversitat de València - Estudi General / IATAexternal researcher - mixed centre
MARIA CARMEN MACIAN ROVIRACollaboratorUniversitat de València
LAURA LÓPEZ OCAÑACollaboratorUniversitat de València
AURORA ZUZUARREGUI MIROCollaboratorUniversitat de València
PATRICIA ELIZAQUIVEL BARCENASCollaboratorUniversitat de València
ALBA PEREZ CATALUÑACollaboratorFundación General CSICresearcher
WALTER RANDAZZO -CollaboratorConsejo Superior de Investigaciones Científicastenured scientist
MARIA DESAMPARADOS RUVIRA GARRIGUESCollaboratorUniversitat de València
MARIA DESAMPARADOS RUVIRA GARRIGUESCollaboratorUniversitat de València
 
CNAE:
  • -
  • -
 
Keywords:
  • Bacterias lácticas; identificación; potencial probiótico; bioconservación; biodiversidad
  • Seguridad alimentaria; qPCR; patógenos alimentarios; métodos rápidos; viabilidad; infectividad; virus entéricos