GIUV2015-252

Self-assembly of amphipathic molecules with lipid and polypeptide characteristics, natural or synthetic. Characterisation of membrane structures of biological, biomedical and biotechnological interest, with special interest in pore, fusion and fission structures. Light-induced molecular changes in membranes, natural or synthetic. Design, implementation and applications of molecular switches in membranes.

https://www.uv.es/membiophys/

• Diseño y caracterización de la interacción con membranas lipídicas de péptidos y proteínas con actividad formadora de poro y remodeladora de membranas.
• Descripción de mecanismos de formación de poro, cambios de curvatura y procesos de fusión y fisión de membranas.
• Determinantes moleculares y propiedades físico-químicas responsables a nivel de secuencia de la actividad en membranas de moléculas polipeptídicas.
• Rediseño de moléculas polipeptídicas para el control de su actividad.
• Desarrollo de nueva metodología, experimental y teórica, para el estudio estructural y funcional de actividades de membrana.

• Design and synthesis of membrane-active polipeptides.Design of new polypeptide sequences with membrane activity from native proteins and peptides. Methodologies: Computational analysis of polypeptide structures at various levels. Chemical synthesis of peptides.
• Biophysical characterization of polypeptide-membrane complexes in model systems.Study of the kinetic and thermodynamic properties of membrane-active complexes. Description of the structure of active complexes and their formation mechanisms. Methodology: infrared, UV-Vis, DC and fluorescence spectroscopies. Fluorescence microscopy. AFM nanoscopy. Langmuir monocaps. Molecular simulations. Theoretical diffusion models. Statistical analysis.

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• Institute of Molecular Science (ICMOL)