GIUV2020-481
Parkinson's disease is the second most common neurodegenerative disease, affecting 1% of the population over 65 years of age. The most characteristic histopathological effect of this disease is the loss of dopaminergic neurons in the substantia nigra and the appearance of intracytoplasmic protein aggregates called Lewy bodies, with alpha-synuclein being the most abundant protein in these aggregates. Recent studies have shown that in Parkinson's disease, as in most human neurodegenerative diseases, the Golgi apparatus is fragmented and dispersed as a consequence of alterations in intracellular trafficking. Our research group has extensive experience in the morphofunctional analysis of intracellular transport both in basic aspects and in the development of the nervous system and in pathological conditions. In this line of research we have worked with cellular models of the disease, human necropsy samples and a hemiparkinsonian rat animal model. The main objective of our line of research is the analysis of the cytopathological mechanisms involved in the fragmentation of the Golgi apparatus and in the alterations of intracellular traffic in the dopaminergic neurons affected in this...Parkinson's disease is the second most common neurodegenerative disease, affecting 1% of the population over 65 years of age. The most characteristic histopathological effect of this disease is the loss of dopaminergic neurons in the substantia nigra and the appearance of intracytoplasmic protein aggregates called Lewy bodies, with alpha-synuclein being the most abundant protein in these aggregates. Recent studies have shown that in Parkinson's disease, as in most human neurodegenerative diseases, the Golgi apparatus is fragmented and dispersed as a consequence of alterations in intracellular trafficking. Our research group has extensive experience in the morphofunctional analysis of intracellular transport both in basic aspects and in the development of the nervous system and in pathological conditions. In this line of research we have worked with cellular models of the disease, human necropsy samples and a hemiparkinsonian rat animal model. The main objective of our line of research is the analysis of the cytopathological mechanisms involved in the fragmentation of the Golgi apparatus and in the alterations of intracellular traffic in the dopaminergic neurons affected in this disease by means of high-resolution morphological techniques supported by biochemical and molecular biology techniques.
[Read more][Hide]
[Read more][Hide]
- Estudiar los mecanismos citopatologicos implicados en las alteraciones del trafico intracelular en las neuronas dopaminergicas de la sustancia negra humana de donantes enfermos de Parkinson, en un modelo celular y en la sustancia negra de un modelo animal de enfermedad de Parkinson.
- Analizar los mecanismos citopatologicos implicados en las alteraciones del trafico intracelular en las neuronas dopaminergicas del sistema nervioso enterico utilizando muestras de colon de un modelo animal de la enfermedad de Parkinson.
- Estudiar las bases celulares y moleculares de la fragmentacion del aparato de Golgi en la neurodegeneracion y analizar las alteraciones en la citoarquitectura del aparato de Golgi mediante tecnicas morfologicas de alta resolucion y estudios de ultraestructura.
- Relacionar los cambios cuantitativos de proteinas implicadas en el trafico de membranas en procesos neurodegenerativos con el estudio de su localizacion a nivel ultraestructual mediante crioinmunocitoquimica por microscopia electronica.
- Intracellular Trafficking in Parkinson's Disease.The main objective of our line of research is the analysis of the cytopathological mechanisms involved in the fragmentation of the Golgi apparatus and in the alterations of intracellular trafficking in dopaminergic neurons in Parkinson's disease.
- Study of the Enteric Nervous System in Parkinson's Disease.Recent studies have shown the presence of Lewy bodies within the enteric nervous system (ENS). Early onset gastrointestinal symptomatology prior to motor symptoms in Parkinson's disease has recently raised the possibility that lesions in the ENS may develop early in the course of the disease, prior to the appearance of cytopathology in the substantia nigra neurons, and thus the study of the ENS may help to understand the cytopathology of Parkinson's disease. These data and the ease of obtaining samples from patients by routine colonic biopsies have led to the use of the SNE as a study model in our line of research. Our main objective is to study the dopaminergic neurons of Meissner's and Auerbach's plexuses from both proximal and distal colon samples from a Parkinsonian rat model. The studies are carried out by means of high-resolution morphological analyses.
| Name | Nature of participation | Entity | Description |
|---|---|---|---|
| MÓNICA TOMAS CABALLERO | Director | Universitat de València | |
| Research team | |||
| Emma Martínez Alonso | Collaborator | Universidad de Murcia | pre-tenured lecturer |
| Mireia Cara Esteban | Collaborator | Universitat de València - Estudi General | UVEG PhD student |
| Mª PILAR MARIN MUELA | Collaborator | INSTITUTO DE INVESTIGACION SANITARIA LA FE (VALENCIA) | postdoctoral researcher |
| Jose Angel Martinez Menarguez | Collaborator | Universidad de Murcia | full university professor |
- -
- Human Anatomy and Embryology
- Parkinson, Aparato de Golgi, citopatología, tráfico intracelular, cuerpos de Lewy, Rab GTP-asas, proteínas SNARE, microscopía electrónica, microscopía confocal.
- Parkinson's, colon, enteric nervous system, Meissner's submucosal plexus, Auerbach's myenteric plexus, Golgi apparatus, intracellular trafficking
